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Title: Localization of CYP86B1 in the outer envelope of chloroplasts. Author: Watson CJ, Froehlich JE, Josefsson CA, Chapple C, Durst F, Benveniste I, Coolbaugh RC. Journal: Plant Cell Physiol; 2001 Aug; 42(8):873-8. PubMed ID: 11522915. Abstract: CYP86B1 was cloned from a cDNA library and the protein expressed in E. coli. The protein gave the expected carbon monoxide difference spectrum. Using in vitro import assays with isolated pea chloroplasts, CYP86B1 was shown to be associated with the outer chloroplastic envelope membrane. This study provides the first direct evidence for a chloroplast-localized cytochrome P450-dependent monooxygenase.[Abstract] [Full Text] [Related] [New Search]