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Title: Crystallization and preliminary X-ray analysis of a depressant insect toxin from the scorpion Buthus martensii Karsch. Author: Guan RJ, Xiang Y, Wang M, Li GP, Wang DC. Journal: Acta Crystallogr D Biol Crystallogr; 2001 Sep; 57(Pt 9):1313-5. PubMed ID: 11526329. Abstract: Depressant insect toxins are a distinct group of scorpion neurotoxins for which no three-dimensional structures are yet available. A depressant insect toxin named BmK dITAP3 from the scorpion Buthus martensii Karsch (BmK) has been purified and crystallized. Single crystals of dITAP3 grew in the presence of the detergent CYMAL-6 using the hanging-drop vapour-diffusion method with ammonium sulfate as precipitant. A set of diffraction data to 2.6 A resolution has been collected. Preliminary analysis of the diffraction data indicated that the crystal belonged to space group R3, with unit-cell parameters a = b = 73.29, c = 68.90 A, alpha = beta = 90, gamma = 120 degrees. Assuming two molecules in the asymmetric unit, the estimated solvent content is 53.4%.[Abstract] [Full Text] [Related] [New Search]