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  • Title: A potentialgradient-conductivity-scanner for the investigation of effects leading to buoyancy-driven convection on continuous-flow-electrophoresis.
    Author: Heinrich J, Wagner H.
    Journal: Adv Space Res; 1992; 12(1):385-92. PubMed ID: 11536984.
    Abstract:
    Continuous flow electrophoresis (CFE) is a mild and high-resolving method for the separation of sensitive biomolecules like peptides and proteins. The resolution is decreased by several effects, of which density gradients are important since they lead to buoyancy-driven convection. A tool for the measurement of physical quantities that cause the separation process, a potentialgradient/conductivity-scanner has been developed. With this PC-scanner consecutive potential- and concentration profiles can be measured inside the CFE-chamber. Local current densities and local joule heat yielding density gradients are calculated from the measurement data. With the PC-scanner the influence of gravity on separations by CFE can be evaluated more easily by the knowledge of the thermal and electrical conditions inside the separation slit.
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