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  • Title: Phytochrome regulates GTP-binding protein activity in the envelope of pea nuclei.
    Author: Clark GB, Memon AR, Tong C-G, Thompson GA, Roux SJ.
    Journal: Plant J; 1993; 4(2):399-402. PubMed ID: 11542500.
    Abstract:
    Three GTP-binding proteins with apparent molecular masses of 27, 28 and 30 kDa have been detected in isolated nuclei of etiolated pea plumules. After LDS-PAGE and transfer to nitrocellulose these proteins bind [32P]GTP in the presence of excess ATP, suggesting that they are monomeric G proteins. When nuclei are disrupted, three proteins co-purify with the nuclear envelope fraction and are highly enriched in this fraction. The level of [32P]GTP-binding for all three protein bands is significantly increased when harvested pea plumules are irradiated by red light, and this effect is reversed by far-red light. The results indicate that GTP-binding activity associated with the nuclear envelope of plant cells is photoreversibly regulated by the pigment phytochrome.
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