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  • Title: Stability of gastrointestinal glutathione peroxidase mRNA in selenium deficiency depends on its 3'UTR.
    Author: Wingler K, Müller C, Brigelius-Flohé R.
    Journal: Biofactors; 2001; 14(1-4):43-50. PubMed ID: 11568439.
    Abstract:
    Selenoproteins decrease upon selenium-deprivation according to their hierarchical ranking. Whereas classical glutathione peroxidase (cGPx) responds to decreased selenium supply with a complete loss of protein and a marked reduction of mRNA levels, gastrointestinal glutathione peroxidase (GI-GPx) remains detectable and its mRNA is stable. The impact of the 3'UTR on cGPx and GI-GPx mRNA stability was studied in stably transfected HepG2 cells with combinations of mutually exchanged coding regions and 3'UTRs of human cGPx and GI-GPx. Stability of chimeric mRNAs was measured by competitive RT-PCR. We found that GI-GPx 3'UTR is sufficient to stabilize its own mRNA but not that of cGPx.
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