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  • Title: A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase.
    Author: Uchida H, Morinaga H, Misaki T, Miyazaki T, Uwajima T, Obata T, Endo Y, Matsuda A, Sasaki T.
    Journal: Nucleosides Nucleotides Nucleic Acids; 2001 Sep; 20(9):1647-54. PubMed ID: 11580191.
    Abstract:
    By affinity chromatography with Sepharose coupled to 2'-deoxy-1-beta-D-ribofuranosyl-N4-dodecanoylcytosine, deoxycytidine kinase and cytidine deaminase were purified 1,950- and 2,240-fold, respectively, from Ehrlich carcinoma cells, and their enzyme activities for several deoxycytidine analogs were investigated.
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