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  • Title: Repeated freezing and thawing does not generally alter assay results for several commonly studied reproductive hormones.
    Author: Reyna R, Traynor KD, Hines G, Boots LR, Azziz R.
    Journal: Fertil Steril; 2001 Oct; 76(4):823-5. PubMed ID: 11591421.
    Abstract:
    OBJECTIVE: To determine the effect of repeated freezing and thawing, and storage temperature, on the assay results of commonly measured reproductive hormones, and sex hormone-binding globulin (SHBG) in human serum. DESIGN: Prospective laboratory study. SETTING: Academic medical center. PATIENT(S): Four men and three pregnant women in the third trimester. INTERVENTION(S): Pooled serum from men and pregnant females were frozen at either -20 degrees C or -70 degrees C. Aliquots were then subjected to repeat freeze/thaw cycles, from 1 to 10 times, and assays were performed after the final freeze/thaw cycle. MAIN OUTCOME MEASURE(S): Assay results for eight hormones (FSH, LH, PRL, androstenedione (A), 17alpha-hydroxyprogesterone, P, insulin, and SHBG, as a function of the number of freeze/thaw cycles and storage temperature. RESULT(S): Only SHBG in male serum at -20 degrees C and P in pregnant serum at -70 degrees C showed statistically significant decreases in assay results with repeated freeze/thaw cycles (3.3% and 1.1% per cycle, respectively). All other analytes did not show significant changes as a function of freeze/thaw cycles or storage temperature. CONCLUSION(S): There is no consistent or predictable alteration in the results of SHBG, or the glycoprotein and steroid hormones evaluated, as a function of repeated freeze/thaw or the storage temperature of human serum.
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