These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Effects of nerve growth factor on nitric oxide-mediated neurotoxicity in primary cortical cultures.
    Author: Wu J, Zhang J.
    Journal: Chin Med J (Engl); 1999 May; 112(5):448-51. PubMed ID: 11593517.
    Abstract:
    OBJECTIVE: To observe the effects of nerve growth factor (NGF) on nitric oxide (NO) release and constitutive nitric oxide synthase (cNOS) gene expression in oxygen/glucose deprived cortical neuron cultures. METHODS: Neuron viability was measured to assay NGF effect. The content of NO and the expression of cNOS mRNA were determined by spectrofluorometric method and Northern blot respectively. RESULTS: There were a marked increase of neuronal death and NO release in oxygen/glucose deprived cultures for 24 h. NOS inhibitor, NG-nitro-L-arginine methyl ester (NAME) 100 mumol.L-1 and NGF 50, 100 micrograms.L-1, significantly increased neuronal surviving and decreased NO release. However, NGF (50, 100 micrograms.L-1) had no significant effects on neurotoxicity induced by sodium nitroprusside (SNP) 300 mumol.L-1 in cortical cultures. Hemoglobin (Hb), which binds NO, completely prevented hypoxia/hypoglycemia- or SNP-induced cell death and NO release at 500 mumol.L-1. NGF 100 micrograms.L-1 significantly attenuated cNOS gene expression. CONCLUSION: NO can mediate the neurotoxicity of hypoxia/hypoglycemia, and NGF can protect cortical neurons against oxygen/glucose deprivation-induced toxicity via inhibiting the activity of cNOS and suppressing the release of NO.
    [Abstract] [Full Text] [Related] [New Search]