These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The bacillus cereus toxin: isolation of permeability factor.
    Author: Ezepchuk Yu V, Bondarenko VM, Yakovleva EA, Koryagina IP.
    Journal: Zentralbl Bakteriol Orig A; 1979 Jul; 244(2-3):275-84. PubMed ID: 116438.
    Abstract:
    The Bacillus cereus protein has been obtained from culture fluid in homogenic form as indicated by SDS-disc electrophoresis and immunodiffusion not described before. The protein has a molecular weight of 100000 daltons. Purification was accomplished by the following steps: (1) removal of ballast nitrous components with DE-32 cellulose at pH 7.2; (2) removal of the proteins from the culture filtrate (deluted four times by water) with DE-32 cellulose at pH 8.6; (3) elution by 0.005 M tris-HCl buffer at pH 7.0 containing 0.5 M NaCl; (4) column rechromatography on DE-32 cellulose at pH 8.6. The isolated protein was identified as a vascular permeability factor acording to the bluing zone in rabbit skin tests or to the bluing lung tissue in mice.
    [Abstract] [Full Text] [Related] [New Search]