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  • Title: Aminoisobutyric acid transport in primary cultures of normal adult rat hepatocytes.
    Author: Ayala E, Canonico PG.
    Journal: Proc Soc Exp Biol Med; 1975 Sep; 149(4):1019-22. PubMed ID: 1166067.
    Abstract:
    In contrast to suspensions of freshly isolated hepatic parenchymal cells (HPC), short-term monolayer cultures of HPC displayed properties of active transport for the amino acid analog aminoisobutyric acid (AIB). The uptake of AIB was inhibited by KCN and iodoacetate, failed to occur at 4 degrees, and was stimulated by glucagon. The apparent Km for AIB uptake by cultured HPC was approximately 19 mM. Glucagon did not alter the apparent Km but did increase V.
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