These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Immunochemical quantitaion of the third component of guinea pig complement in fluid phase and bound to cell surfaces.
    Author: Ohanian SH, Borsos T.
    Journal: J Immunol; 1975 Jan; 114(1 Pt 2):326-30. PubMed ID: 1167878.
    Abstract:
    A method is described for the determination of the absolute amount of guinea pig (GP) C-3 bound to cell surfaces. The test is based on the inhibition of anti-C-3 by C-3. The C-3 to be measured is added to a standard amount of anti-C-3 antibody and the residual activity is determined by its capacity to sensitize EC43-gp for lysis by GPC. By comparing the observed lysis with inhibition-of-lysis curves obtained with known C-3 antigen, the amount of test antigen can be determined. The method is simple, rapid, and reproducible and can detect nanogram amounts of C-3. The C-3 content of six normal guinea pig sera was found to be 1365 plus or minus 56 mug/ml (ranges 1120 to 1510 mug/ml) by the inhibition method described in this paper. Guinea pig hepatoma cells, line-1 and line-10, harvested from the peritoneal cavity of guinea pigs contained approximately 6.7 x 10-3 to 6.2 x 10-4 GPC-3 per cell whereas tumor cells sensitized with anti-Forssman or specific anti-tumor antibody contained from 1 to 9 x 10-5 C-3 per cell. Susceptibility to killing by antibody and GPC could not be correlated with the amount of GPC-3 bound to the surfaces of the tumor cells. Comparisons made between the amounts of C-3 bound to antibody-sensitized sheep erythrocytes and nucleated cells suggest there is a fundamental quantitative difference in the fixation of selected C components.
    [Abstract] [Full Text] [Related] [New Search]