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  • Title: Degradation of 2-methylnaphthalene by Pseudomonas sp. strain NGK1.
    Author: Sharanagouda U, Karegoudar TB.
    Journal: Curr Microbiol; 2001 Dec; 43(6):440-3. PubMed ID: 11685513.
    Abstract:
    Pseudomonas sp. strain NGK1, a soil bacterium isolated by naphthalene enrichment from biological waste effluent treatment, capable of utilizing 2-methylnaphthalene as sole source of carbon and energy. To deduce the pathway for biodegradation of 2-methylnaphthalene, metabolites were isolated from the spent medium and identified by thin-layer chromatography and high-performance liquid chromatography. The characterization of purified metabolites, oxygen uptake studies, and enzyme activities revealed that the strain degrades 2-methylnaphthalene through more than one pathway. The growth of the bacterium, utilization of 2-methylnaphthalene, and 4-methylsalicylate accumulation by Pseudomonas sp. strain NGK1 were studied at various incubation periods.
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