These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Different secretory vesicles can be involved in depolarization-evoked exocytosis.
    Author: Lukyanetz EA.
    Journal: Biochem Biophys Res Commun; 2001 Nov 09; 288(4):844-8. PubMed ID: 11688985.
    Abstract:
    The relationship between Ca(2+) influx through voltage-activated Ca(2+) channels, resting intracellular Ca(2+) level (Ca(i)) and Ca(2+)-dependent exocytosis was studied in bovine adrenal chromaffin cells by using patch-clamp, capacitance, and fluorescent measurements. It was established that depolarization-induced exocytosis passed over two steps, both of which linearly depend on Ca(i). At Ca(i) lying below critical point (200-300 nM) the slope of the relationship was 4.43 and at Ca(i) exceeding the critical point the slope was equal to 31.63. The vesicular mechanism describing experimental two-step dependence of exocytosis on intracellular Ca(2+) (Ca(i)) is proposed. According to the model at Ca(i) below critical point only small-sized vesicles fuse with plasma membrane, whereas at higher Ca(i), larger vesicles started to fuse.
    [Abstract] [Full Text] [Related] [New Search]