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  • Title: Synthesis of immobilized flavin derivatives and their use in purification of chicken egg-white ovoflavoprotein.
    Author: Blankenhorn G, Osuga DT, Lee HS, Feeney RE.
    Journal: Biochim Biophys Acta; 1975 Apr 29; 386(2):470-8. PubMed ID: 1169970.
    Abstract:
    Affinity adsorbents for flavoproteins were prepared by the covalent attachment of polyacrylamide and agarose to flavin derivatives linked through position N(3) of the flavin nucleus. 3-Carboxymethyl-FMN covalently linked to aminoalkyl substituted agarose was successfully used for the separation and purification of the apo form of the ovoflavoprotein from chicken egg white. High yields and high purities were achieved by two different isolation procedures employing the affinity adsorbent.
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