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  • Title: Miniaturization of capillary isoelectric focusing.
    Author: Wu XZ, Sze SK, Pawliszyn J.
    Journal: Electrophoresis; 2001 Oct; 22(18):3968-71. PubMed ID: 11700727.
    Abstract:
    Miniaturization of whole-column imaging capillary isoelectric focusing (CIEF) is discussed. A 1.2 cm capillary was used as a separation column for CIEF. The experimental results for the analysis of two pI markers and the protein myoglobin showed that good CIEF separation results could be obtained. Secondly, a light-emitting diode (LED) was used as the light source for the whole-column absorbance imaging detection. The focusing of both the pI markers and myoglobin were observed with the LED light source. The whole-column imaging CIEF instrument was simplified and miniaturized by the use of the LED. Further developments are also discussed.
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