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  • Title: Specific tritium labeling of cerebrosides at the 3-positions of erythro-sphingosine and threo-sphingosine.
    Author: Iwamori M, Moser HW, Kishimoto Y.
    Journal: J Lipid Res; 1975 Jul; 16(4):332-6. PubMed ID: 1170270.
    Abstract:
    Cerebrosides containing either threo- or erythro-[3-3-H]sphingosine were synthesized by a new procedure. Glucopyranosyl or galactopyranosyl ceramides were converted to their 3-keto derivatives with 2,3-dichloro-5,6-dicyanobenzoquinone and reduced with 3-H-labeled sodium borohydride. The resulting tritiated cerebrosides, which contained erythro- and threo-sphingosines in the ratio of 84:16, were deacylated with butanol-KOH, and the erythro- and threo-psychosines were separated by silica gel column chromatography and reacylated with lignoceroyl chloride.
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