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  • Title: [Effect of expression of c-myc gene on hyperplasia of smooth muscle cells in vein graft in rat].
    Author: Wang X, Zhang Q, Wang B.
    Journal: Zhonghua Yi Xue Za Zhi; 1999 May; 79(5):381-3. PubMed ID: 11715481.
    Abstract:
    OBJECTIVES: To analyse effect of expression of c-myc gene on hyperplasia of smooth muscle cells in vein graft and evaluate the effect of c-myc gene on intimal hyperplasia of vein grafts. METHODS: We transplanted the autologous internal jugular vein on iliac artery by end to end anastomosis in rat. The vein grafts were harvested at 30 min, 2 h, 6 h, 2 days, 1 week, 2 weeks and 4 weeks after grafting and were rapidly frozen in liquid nitrogen. Proteins of PCNA and c-myc were detected in the different time with method of immunohistochemical staining (SP) and c-myc mRNA with in situ hybridization. Staining result was measured by microscope (400X). RESULTS: Freshly harvested veins did not demonstrate PCNA, c-myc protein production and c-myc mRNA. Staining for c-myc mRNA was significantly higher at 2 h, 6 h compared with that at 30 min (P < 0.01). Staining for PCNA, c-myc protein production peaked at 1 week (44.8 +/- 8.2)%, (16.2 +/- 3.2)%, respectively) and was significantly higher than that at 6 h (3.2 +/- 0.7)%, (4.1 +/- 0.9)%, respectively) (P < 0.01). CONCLUSION: c-myc is expressed early after experimental vein grafting, with peak at 1 week paralleled with PCNA. This occurs during a period of maximal SMC proliferation, and increased expression of PCNA. Expression of c-myc gene may contribute to the induction and regulation of SMC proliferation.
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