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  • Title: [Preliminary study on the immune response in C57BL/6 mice vaccinated by nucleic acid encoding paramyosin of Chinese S. japonicum].
    Author: Zhou S, Liu S, Song G.
    Journal: Zhonghua Yi Xue Za Zhi; 1999 Nov; 79(11):848-51. PubMed ID: 11715491.
    Abstract:
    OBJECTIVE: To study the characteristics of immune response in C57 BL/6 mice by nucleic acid encoding full-length paramyosin of Chinese S. japonicum. METHODS: Total RNA was isolated from adult Chinese S. japonicum using TRIzol reagent. The full-length cDNA encoding paramyosin of Chinese S. japonicum was amplified by RT-PCR and cloned into expression vector pcDNA/Amp (pCMV-SjC97), which was intramuscularly injected into C57 BL/6 mice. The mice were vaccinated with DNA the three-week interval for a total of three inoculations. The specific anti-SjC97 IgM, IgG and IgE antibodies and IgG subclasses were determined by ELISA test. RESULTS: The pCMV-SjC97 vaccine induced specific anti-SjC97 IgM and IgG antibodies. The antibody response correlated well with the administered amount of pCMV-SjC97. The IgG subclasses, predominantly IgG2a and IgG2b, were observed in the mice vaccinated with pCMV-SjC97, but no IgG1 and IgG3 responses were seen. CONCLUSION: The strong immune response, especially Th1 type, can be induced in the mice with pCMV-SjC97 vaccine.
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