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  • Title: [Cloning and sequencing of the VL and VH genes from the anti-vascular endothelial growth factor McAb hybridoma cell line].
    Author: Ran Y, Yang Z, Wang G, Dong Z.
    Journal: Zhongguo Yi Xue Ke Xue Yuan Xue Bao; 1998 Dec; 20(6):423-7. PubMed ID: 11717933.
    Abstract:
    OBJECTIVE: The light chain variable region (VL) and heavy chain variable region (VH) genes from the anti-VEGF165 McAb hybridoma cell line were cloned for construction of it's chimeric antibody. METHODS: The VL and VH genes were amplified from the total RNA of the VmD11 hybridoma with RT-PCR and cloned into vectors, and then analyzed by sequencing. RESULTS: The 320 bp VL gene belong to mouse kappa light chain subgroup V, the 354 bp VH gene belong to mouse heavy chains subgroup I (B). CONCLUSIONS: The nucleotide sequence analysis indicated that the cloned genes coded the light and heavy chain variable region of mouse respectively.
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