These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Detection of DNA point mutations and mRNA expression levels by rolling circle amplification in individual cells.
    Author: Christian AT, Pattee MS, Attix CM, Reed BE, Sorensen KJ, Tucker JD.
    Journal: Proc Natl Acad Sci U S A; 2001 Dec 04; 98(25):14238-43. PubMed ID: 11724932.
    Abstract:
    Rolling circle amplification has been useful for detecting point mutations in isolated nucleic acids, but its application in cytological preparations has been problematic. By pretreating cells with a combination of restriction enzymes and exonucleases, we demonstrate that rolling circle amplification in situ can detect gene copy number and single base mutations in fixed cells with efficiencies up to 90%. It can also detect and quantify transcribed RNA in individual cells, making it a versatile tool for cell-based assays.
    [Abstract] [Full Text] [Related] [New Search]