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  • Title: A new and simple procedure for the evaluation of the association of surfactants to proteins.
    Author: Lissi E, Abuin E, Lanio ME, Alvarez C.
    Journal: J Biochem Biophys Methods; 2002 Jan 04; 50(2-3):261-8. PubMed ID: 11741713.
    Abstract:
    A new and simple method useful for the evaluation of the association of surfactants to proteins is proposed. The method is based on an analysis of the effect promoted by surfactant addition upon the fluorescence intensity of the intrinsic tryptophan chromophore and its dependence with protein concentration. The proposed methodology is applied to quantify the binding of an anionic (sodium dodecylsulfate), a zwitterionic (N-hexadecyl-N,N'-dimethyl-3-ammonio-1-propane-sulfonate) and a neutral (Triton X-100, reduced) surfactant to bovine serum albumin (BSA).
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