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  • Title: A method for determination of lecithin/sphingomyelin ratio in amniotic fluid.
    Author: Singh EJ, Zuspan FP.
    Journal: Arch Gynakol; 1975 Jul 29; 218(3):169-71. PubMed ID: 1174303.
    Abstract:
    A method is described for the evaluation of amniotic fluid lecithin/sphingomyelin (L/S) ratios. The acetone precipitation step is eliminated, resulting in higher concentrations of phospholipids. Silica gel-impregnated glass fiber papers are used for rapid chromatography. For spot detection, a bismuth reagent is used which is specific for lecithin and sphingomyelin. The method is simple, inexpensive, rapid and a reliable indicator of the L/S ratio, and useful in laboratories that are not equipped with a densitometer.
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