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  • Title: Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced.
    Author: van Hijum SA, Bonting K, van der Maarel MJ, Dijkhuizen L.
    Journal: FEMS Microbiol Lett; 2001 Dec 18; 205(2):323-8. PubMed ID: 11750822.
    Abstract:
    Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme. When incubated with sucrose the enzyme produced a levan that is identical to that produced by Lb. reuteri strain 121 cells.
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