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  • Title: The biotin switch method for the detection of S-nitrosylated proteins.
    Author: Jaffrey SR, Snyder SH.
    Journal: Sci STKE; 2001 Jun 12; 2001(86):pl1. PubMed ID: 11752655.
    Abstract:
    Many of the effects of nitric oxide are mediated by the direct modification of cysteine residues resulting in an adduct called a nitrosothiol. Here, we describe a novel method for detecting proteins that contain nitrosothiols. In this three-step procedure, nitrosylated cysteines are converted to biotinylated cysteines. Biotinylated proteins can then be detected by immunoblotting or can be purified by avidin-affinity chromatography. We include examples of the detection of S-nitrosylated proteins in brain lysates after in vitro S-nitrosylation, as well as the detection of endogenous S-nitrosothiols in selected neuronal proteins.
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