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  • Title: Characterization of nucleoside diphosphate kinase from moderately halophilic eubacteria.
    Author: Yonezawa Y, Tokunaga H, Ishibashi M, Tokunaga M.
    Journal: Biosci Biotechnol Biochem; 2001 Oct; 65(10):2343-6. PubMed ID: 11758938.
    Abstract:
    Nucleoside diphosphate kinase was purified to apparent homogeneity from naturally isolated moderately halophilic eubacteria by ATP-agarose and phenyl-5PW column chromatographies. The molecular mass of this enzyme was 15 kDa by time-of-flight mass-spectrometry. This protein showed anomalous mobility on SDS-PAGE which is typical of a halophilic protein. It was stable and active over a wide range of salt concentrations, from 0 to 4.0 M NaCl.
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