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  • Title: [Study on anti-HBV effects by antisense oligodeoxynucleotides in vitro].
    Author: Liu S, Sun W, Cao Y.
    Journal: Zhonghua Yu Fang Yi Xue Za Zhi; 2001 Sep; 35(5):338-40. PubMed ID: 11769637.
    Abstract:
    OBJECTIVE: To study the inhibition effect of anti-HIV oligodeoxy-nucleotides on HBV X gene. METHODS: Three antisense phosphorothioate oligodeoxy-nucleotides(AsON) complementary to the initiator of X, DR2, EN II respectively, were synthesized and tested for their anti-HBV activity on HepG2.2.15 cells with ELISA methods. RESULTS: When the best effect concentration of AsON was 10 mumol/L, the inhibition rates on HBsAg of three AsON were 57%, 60% and 52% respectively, and on HBeAg were 56%, 45% and 56% respectively. There were two inhibition peaks at different times. There was no inhibition effect treated by random control (< 12%). Using the 3-(4,5-dimethythiazol-xyl)-2,5-diphnyl tetrazolium bromide method, there was no cytotoxicity at 40 mumol/L of AsON. CONCLUSION: AsONs on three key region of X gene were effective drugs to inhibit the expression of HBV.
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