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  • Title: [Quantitative detection of Mycobacterium tuberculosis DNA in peripheral blood from patients with pulmonary tuberculosis by AmpliSensor-PCR technique].
    Author: Tan Y, Li Y, Zhang Y.
    Journal: Zhonghua Jie He He Hu Xi Za Zhi; 1999 Aug; 22(8):481-3. PubMed ID: 11776564.
    Abstract:
    OBJECTIVE: To evaluate the clinical value of the quantitative detection of Mycobacterium tuberculosis DNA by Amplisensor-PCR in peripheral blood from patients with pulmonary tuberculosis. METHODS: The model of Mycobacterium tuberculosis DNA in blood was made by QIAamp and AcuPure methods, quantitative detection of Mycobacterium tuberculosis DNA by AmpliSensor-PCR technique and the results were compared with IS6110 single-tube nested PCR (SN-PCR). RESULTS: The peripheral blood from 200 patients with pulmonary tuberculosis were detected respectively by using AmpliSensor-PCR and IS6110 SN-PCR methods. The positive rates of Mycobacterium tuberculosis DNA were 60.5% and 63.5% respectively, and that from 85 patients with non-pulmonary tubeculosis were 4.7%, 8.2%. CONCLUSIONS: AmpliSensor-PCR method showed higher sensitivity and specificity in detecting the Mycobacterium tubeculosis DNA from peripheral blood. The variation of the TB-DNA may be useful in evaluating the prognosis of the patients.
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