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  • Title: [Expression and reversion of drug resistance- and apoptosis-related genes of a DDP-resistant lung adenocarcinoma cell line A549DDP].
    Author: Wang J, Liu X, Wu M.
    Journal: Zhonghua Zhong Liu Za Zhi; 1999 Nov; 21(6):422-6. PubMed ID: 11776616.
    Abstract:
    OBJECTIVE: To study the expression of drug resistance- and apoptosis-related genes of A549DDP cells as compared to the parental cell line A549, and its reversion by antisense s-oligodeoxynucleotide (S-ODN) of the differentially expressed genes. METHODS: Sense and antisense S-ODN were transferred into A549DDP cells by lipofectin. Expression of genes related to drug resistance and apoptosis was examined by RT-PCR, immunocytochemistry and flow cytometry. Apoptosis was identified by DNA electrophoresis and TUNEL, and cell growth by MTT uptake. RESULTS: The expression of bcl-2 was positive and that of MRP at mRNA and protein levels was increased in A549DDP cells compared to A549 cells. MDR1, c-myc and TOPO II were similarly expressed in the two cell lines. Both cell lines were negative for c-erbB-2 expression. In A549DDP cells, the expression of bcl-2 and MRP was significantly inhibited by respective antisense S-ODN. Antisense S-ODN could also significantly inhibit proliferation of A549DDP cells, and promote cell apoptosis by reducing its resistance to cisplatin. CONCLUSION: Bcl-2 and MRP genes are responsible for the induced resistance of A549DDP cells to cisplatin.
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