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  • Title: [Glutathione synthesis inhibitor enhances arsenic trioxide-induced apoptosis].
    Author: Shen Y, Chen G, Cai X.
    Journal: Zhonghua Zhong Liu Za Zhi; 1999 Jul; 21(4):259-61. PubMed ID: 11776809.
    Abstract:
    OBJECTIVE: To illustrate the possible role of thiols (SH-groups) in arsenic trioxide (As2O3)-induced apoptosis. METHODS: Leukemia (NB4, HL60, U937) and lymphoma (Jurkat and Namalwa) cell lines were treated with As2O3 together with buthionine sulfoximine (BSO), a selective inhibitor of gamma-glutamylcysteine synthetase. The mitochondrial transmembrane potentials (delta psi m) and the percentage of cells in apoptosis were measured by means of flow cytometry. RESULTS: One mmol/L BSO significantly enhanced As2O3-induced delta psi m collapse and apoptosis in NB4 cells. One mumol/L As2O3 did not induce the delta psi m collapse and apoptosis in Namalwa, HL60, U937 and Jurkat cells, while these effects were induced significantly by the co-treatment of 1 mumol/L As2O3 and 1 mmol/L BSO. CONCLUSION: Thiols are important chemosensors of As2O3-induced delta psi m collapse and apoptosis.
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