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  • Title: [NF-kappa B activation and the inhibitory effect of dexamethasone in experimental asthmatic guinea pigs].
    Author: Zhang Y, Ouyang N, Xu J.
    Journal: Zhonghua Jie He He Hu Xi Za Zhi; 2000 Jul; 23(7):406-9. PubMed ID: 11778505.
    Abstract:
    OBJECTIVE: To investigate the role of NF-kappa B in pathogenesis of asthma. METHODS: Electrophoresis mobility shift assay and supershift assay were used to analyze the expression of NF-kappa B activation and its components. RESULTS: Nuclear extracts prepared from the thoracic blood mononuclear cells and the whole lung tissue, respectively in OVA-sensitized and challenged guinea pigs, both displayed stronger DNA-binding activity at 2 h timepoint (244.1 +/- 2.4; 176.0 +/- 4.0) comparing to the control (167.0 +/- 4.6; 67.3 +/- 2.5), and the activity peaked at 6 h timepoint (294.7 +/- 3.2; 282.3 +/- 6.8) and continued till 12 h timepoint (200.9 +/- 1.2; 110.8 +/- 1.0). The activated NF-kappa B contained p50. Dexamethasone suppressed the NF-kappa B activation (235.4 +/- 3.3; 104.6 +/- 8.4) in the nuclear extracts of the Blood mononuclear cells and the lung tissue, respectively. CONCLUSIONS: This result showed that NF-kappa B were possibly through transcriptionally regulating the expression of many important inflammatory proteins. The dexamethasone could inhibit the activation of NF-kappa B in the allergic model, possibly through which the antiinflammatory action was exerted.
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