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  • Title: In-vitro cultivation of normal human oral keratinocytes.
    Author: Zhou Z, Zhou H, Shang Q, Cao Y.
    Journal: Chin Med J (Engl); 2001 Jul; 114(7):731-4. PubMed ID: 11780338.
    Abstract:
    OBJECTIVE: To establish a method for culturing normal human oral keratinocytes. METHODS: Specimens obtained from healthy humans undergoing oral surgery were dissociated into single cell suspensions by dispase and trypsin. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope. Cytokeratins were shown by immunohistochemistry. RESULTS: Cells could be maintained in culture up to 4-5 passages or 30-50 days. Electron microscope revealed that there were desmosomes and tonofibrils in the oral keratinocytes. The cells showed positive staining for cytokeratin antibody. CONCLUSION: Human oral keratinocytes have been successfully grown in serial culture.
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