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  • Title: [Induction of fluoride-resistant mutant of S. mutans and the measurement of its acidogenesis in vitro].
    Author: Sheng J, Liu Z.
    Journal: Zhonghua Kou Qiang Yi Xue Za Zhi; 2000 Mar; 35(2):95-8. PubMed ID: 11780495.
    Abstract:
    OBJECTIVE: To induce fluoride-resistant mutant of S. mutans in vitro and find out the differences of cariogenicity between them. METHODS: Fluoride-resistant strain Ingbritt-FR was obtained by subculturing S. mutans Ingbritt to TSA and TSB containing different concentrations of fluoride. Lactic production and terminal pH of media were measured by gas chromatography method. RESULTS: 1. The stable fluoride-resistant mutant of S. mutans Ingbritt had been induced successfully in vitro; 2. Lactic production of Ingbritt-FR was significantly more than its parent strain at various initial pH values with 0.5 and 5.0 mmol/L fluoride whereas when no fluoride existed, there were two situations: the acid production was more than S. mutans Ingbritt at initial pH above 5.0 while the result was opposite when pH was 5.0 and below. CONCLUSION: The fluoride-resistant strain Ingbritt-FR had a stronger cariogenic potential than its parent strain S. mutans Ingbritt in physiological plaques.
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