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  • Title: [Serial cultivation of normal human oral keratinocytes].
    Author: Zhou H, Zhou Z, Shang Q.
    Journal: Zhonghua Kou Qiang Yi Xue Za Zhi; 2000 Nov; 35(6):455-7. PubMed ID: 11780534.
    Abstract:
    OBJECTIVE: To establish a method of culturing normal human oral keratinocytes. METHODS: Specimens obtained from healthy humans undergoing oral surgery were dissociated by Dispase and trypsin into a single cell suspension. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope (EM). Cytokeratins were showed by immunohistochemistry. RESULTS: The cells could be maintained in culture up to 4-5 passages, or 30-50 days. EM revealed that there were desmosomes and tonofibrils in the culture cells. The cells showed positive staining for cytokeratin antibody. CONCLUSIONS: The culture technique for growing normal human oral keratinocytes has been established.
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