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  • Title: [The effect of immune bovine whey on cell-associated glucosyltransferase activity of Streptococcus mutans].
    Author: Chen W, Fan M, Bian Z.
    Journal: Zhonghua Kou Qiang Yi Xue Za Zhi; 2000 Nov; 35(6):467-9. PubMed ID: 11780538.
    Abstract:
    OBJECTIVE: To study the effect of immune bovine whey on cell-associated glucosyltransferase (GTF) activity of S. mutans MT8148. METHODS: The immune milk was collected from cows immunized with cell-associated GTF overexpression strain B-29-33 of S. mutans MT8148. The control milk was from non-immunized cows. The immune absorbed whey was gotten from immune bovine whey which was absorbed with lyophilized Formalin-killed B-29 whole cells. Three kinds of whey were subdivided into three groups: 50 microliters, 70 microliters, 90 microliters. The content of insoluble glucan was estimated colorimetrically by anthrone method. RESULTS: The control bovine whey had an enhancing GTF activity (407.00%-485.62%). The immune absorbed whey inhibited partly the enhancing GTF activity (208.74%-273.00%). The immune whey inhibited significantly the GTF activity (70.24%-38.62%) and the inhibition showed a tendency to depend on doses. CONCLUSIONS: The immune bovine whey inhibits significantly the cell-associated GTF activity of S. mutans MT8148.
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