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  • Title: Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length.
    Author: Schott DH, Collins RN, Bretscher A.
    Journal: J Cell Biol; 2002 Jan 07; 156(1):35-9. PubMed ID: 11781333.
    Abstract:
    Myosins are molecular motors that exert force against actin filaments. One widely conserved myosin class, the myosin-Vs, recruits organelles to polarized sites in animal and fungal cells. However, it has been unclear whether myosin-Vs actively transport organelles, and whether the recently challenged lever arm model developed for muscle myosin applies to myosin-Vs. Here we demonstrate in living, intact yeast that secretory vesicles move rapidly toward their site of exocytosis. The maximal speed varies linearly over a wide range of lever arm lengths genetically engineered into the myosin-V heavy chain encoded by the MYO2 gene. Thus, secretory vesicle polarization is achieved through active transport by a myosin-V, and the motor mechanism is consistent with the lever arm model.
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