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  • Title: [Relationship among telomerase activity, telomerase RNA and telomerase catalytic subunit gene expression and their significance in non small-cell lung cancer].
    Author: Wang J, Liu X, Jiang W.
    Journal: Zhonghua Zhong Liu Za Zhi; 2001 Jul; 23(4):289-92. PubMed ID: 11783109.
    Abstract:
    OBJECTIVE: To study telomerase activity (TA), telomerase RNA (hTR) and telomerase catalystic subunit (hTRT/hEST2) code gene expression in non-small cell lung cancer (NSCLC), their relationship and significance. METHODS: Telomerase repeat amplification protocol PCR (TRAP-PCR) for TA, RT-PCR for hTR and in situ hybridization for hTRT/hEST2 mRNA expression were performed. RESULTS: Positive rates of TA, hTR and hTRT/hEST2 were 68.4%(26/38), 51.7%(32/58) and 74.2%(43/58) in NSCLC. But, the adjacent and benign lung tissues were negative for expression of TA, hTR and hTRT/hEST2. A statistically significant positive correlation between TA and hTRT/hEST2 was observed(r = 0.84, P = 0.01), though there was no statistically significant positive association between TA and hTR (r = 0.16, P = 0.23). The median survivals of TA and hTRT/hEST2 positive groups (10.4 months, 7.5 months) were shorter than the negative ones (13.5 months, 14.7 months). However, only the hTRT/hEST2 group was found to show statistical significance to be an independent prognostic factor by multivariate Cox regression analysis. CONCLUSION: Telomerase activity, hTR expression and hTRT/hEST2 are frequently detected at higher levels in NSCLC than the pericancerous normal tissues. There is a positive correlation between TA and hTRT/hEST2, both of which are markers of poor magnificent phenotype of NSCLC. The hTRT/hEST2 has an independent prognostic significance.
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