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  • Title: [The expression of bombesin receptor in gastric cancer].
    Author: Sun T, Cheng Y, Hou M, Li X, Chen Y.
    Journal: Zhonghua Nei Ke Za Zhi; 1999 Feb; 38(2):85-7. PubMed ID: 11798630.
    Abstract:
    OBJECTIVE: To observe the expression of receptor mRNA and its protein in SGC 7901 cells. METHODS: (1) RT-PCR and Southern blot hybridization were performed for detection of gastrin releasing peptide GRP-R mRNA; (2) Covalent cross-linking study with biotinyl-bombesin and enhanced chemoluminescence (ECL) was performed to identify GRP-R at protein levels in SGC 7901 cells. RESULTS: (1) It is shown that the molecular weight of the cDNA was consistent with that of expected. Southern blot was then used to further prove that specific GRP-R mRNA was expressed by SGC 7901. (2) GRP-R protein was shown by covalent cross-linking in SGC 7901 and the molecular weight of bombesin/GRP-R in SGC 7901 cells was about 75000. CONCLUSION: By using RT-PCR, Southern blot and covalent cross-linking techniques, it was demonstrated that SGC 7901 cells can express bombesin receptor mRNA and protein. The results of this study provides a basis for further researches on bombesin intracellular signal transmission pathway and the role of bombesin and bombesin receptor antagonists in oncological research.
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