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  • Title: Composition and functional characterization of the yeast spliceosomal penta-snRNP.
    Author: Stevens SW, Ryan DE, Ge HY, Moore RE, Young MK, Lee TD, Abelson J.
    Journal: Mol Cell; 2002 Jan; 9(1):31-44. PubMed ID: 11804584.
    Abstract:
    Pre-mRNA introns are spliced in a macromolecular machine, the spliceosome. For each round of splicing, the spliceosome assembles de novo in a series of ATP-dependent steps involving numerous changes in RNA-RNA and RNA-protein interactions. As currently understood, spliceosome assembly proceeds by addition of discrete U1, U2, and U4/U6*U5 snRNPs to a pre-mRNA substrate to form functional splicing complexes. We characterized a 45S yeast penta-snRNP which contains all five spliceosomal snRNAs and over 60 pre-mRNA splicing factors. The particle is functional in extracts and, when supplied with soluble factors, is capable of splicing pre-mRNA. We propose that the spliceosomal snRNPs associate prior to binding of a pre-mRNA substrate rather than with pre-mRNA via stepwise addition of discrete snRNPs.
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