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  • Title: Liquid chromatographic/mass spectrometric detection of the 3-(3-hydroxyalkanoyloxy) alkanoic acid precursors of rhamnolipids in Pseudomonas aeruginosa cultures.
    Author: Lépine F, Déziel E, Milot S, Villemur R.
    Journal: J Mass Spectrom; 2002 Jan; 37(1):41-6. PubMed ID: 11813309.
    Abstract:
    A series of pseudomolecular and fragment ions attributed to 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs) were detected by liquid chromatography/mass spectrometry among the rhamnolipids observed in a Pseudomonas aeruginosa strain 57RP supernatant. The fragmentation mechanism leading to the formation of the fragment ions was determined by a deuterium exchange experiment and by using a standard HAA mixture obtained from the mild acidic hydrolysis of rhamnolipids of known composition. The structure and the response factor of these free HAAs were determined. The HAAs relative composition differs between free HAAs and those present in rhamnolipids, the former being enriched in lower molecular mass congeners and depleted in the heavier ones. Within an isomeric pair, the isomer with the shortest 3-hydroxyalkaloyl residue at the hydroxyl end was more abundant than the one with the heavier 3-hydroxyalkaloyl acid at this position, and the ratios of their relative abundances were similar for free HAAs and those in rhamnolipids. Experiments with deuterium-labeled rhamnolipids demonstrated that free HAAs are part of a pool used for rhamnolipid biosynthesis and are not rhamnolipid degradation products.
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