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  • Title: Supercritical fluid extraction and high-performance liquid chromatography-diode array-electrochemical detection of signature redox compounds from sand and soil samples.
    Author: Lang Q, Cheng IF, Wai CM, Paszczynski A, Crawford RL, Barnes B, Anderson TJ, Wells R, Corti G, Allenbach L, Erwin DP, Assefi T, Mojarradi M.
    Journal: Anal Biochem; 2002 Feb 15; 301(2):225-34. PubMed ID: 11814293.
    Abstract:
    A supercritical fluid extraction procedure and a chromatographic separation/detection method were developed for the detection of Earth-based microorganisms. After microbes in a sand or a soil sample were hydrolyzed in a diluted NH(4)OH/acetone solution, several redox compounds from bacteria could be effectively extracted with trimethylamine-modified supercritical CO(2) at 35 degrees C and 300 atm. These signature redox-active compounds were separated by a reversed-phase HPLC column in an ion-pair mode and then monitored with a diode array detector and an electrochemical detector. The analytical results demonstrated the feasibility of using the reported techniques to detect the chemical signature of life in barren desert sand samples.
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