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  • Title: Development of a chromatographic method for the determination of saquinavir in plasma samples of HIV patients.
    Author: Campanero MA, Escolar M, Arangoa MA, Sádaba B, Azanza JR.
    Journal: Biomed Chromatogr; 2002 Feb; 16(1):7-12. PubMed ID: 11816005.
    Abstract:
    A simple, sensitive and reproducible high-performance liquid chromatographic method for detecting and quantifying saquinavir in human plasma is described. Verapamil was used as internal standard. The method employes a single liquid-liquid extraction step with tert-butil methyl ether followed by chromatography on a Lichrospher 60 Select B C8 reversed-phase column. Ultraviolet detection was used to identify the compounds of interest. The quantitation limit of saquinavir was 1 ng/mL and only 0.5 mL of plasma sample was required for the determination. The average saquinavir recoveries over a concentration range of 2.5-500 ng/mL ranged from 86 to 95%. Precision and accuracy did not exceed 5%.
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