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  • Title: Normal pregnancies and live births after autograft of frozen-thawed hemi-ovaries into ewes.
    Author: Salle B, Demirci B, Franck M, Rudigoz RC, Guerin JF, Lornage J.
    Journal: Fertil Steril; 2002 Feb; 77(2):403-8. PubMed ID: 11821105.
    Abstract:
    OBJECTIVE: To evaluate long-term outcome of autotransplantation of cryopreserved hemi-ovaries into ewes. DESIGN: Animal study. SETTING: University fertility center, Hospices Civils de Lyon; and Ecole Nationale Vétérinaire de Lyon. PATIENT(S): Grivette ewes. INTERVENTION(S): Six hemi-ovaries from 6 ewes aged 6 to 12 months were frozen with a slow cooling protocol using 2 M of dimethyl sulfoxide as cryoprotectant. After dissection of the medulla, the hemi-ovarian cortex was stored at -196 degrees C in liquid nitrogen. Freezing procedure was performed with a programmable freezer. Semiautomatic seeding was performed before crystallization. Four to 6 weeks after the first laparotomy, the left ovary was removed and the frozen-thawed hemi-ovary was sutured. MAIN OUTCOME MEASURE(S): Mean plasma concentrations of FSH, LH, and progesterone after autotransplantation of frozen-thawed hemi-ovary. Ultrasonography was done to confirm pregnancy. Blood samples were collected weekly to measure FSH, LH, and progesterone. After the first birth, the autografted ovary was removed for histologic examination. RESULT(S): Plasma progesterone concentration increased in a regular manner in all ewes except one 4 weeks after the graft. Concentrations of FSH and LH did not reach the menopausal level. Four pregnancies occurred, from which 6 lambs were born. The first delivery of a normal lamb occurred after 135 days of gestation; the lamb died immediately after birth. The second delivery of two normal lambs occurred after 130 days of gestation. A caesarean section was performed on the third pregnant ewe the 110th days of gestation because the ewe had a vaginal prolapsus. The two normal lambs and the ewe died after surgery. The fourth birth of a normal lamb occurred after 132 days of gestation. Histologic examination of the grafted frozen-thawed ovary showed a regressing corpus luteum and few primordial and antral follicles. CONCLUSION(S): These four pregnancies in a ewe model may indicate that women who undergo preservation of their ovaries before chemotherapy or radiotherapy can have successful pregnancy.
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