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  • Title: Peptidases affecting recombinant protein production by Streptomyces lividans.
    Author: Yun SI, Yahya AR, Malten M, Cossar D, Anderson WA, Scharer JM, Moo-Young M.
    Journal: Can J Microbiol; 2001 Dec; 47(12):1137-40. PubMed ID: 11822841.
    Abstract:
    The influence of peptidases on human interleukin-3 (rhIL-3) production by a recombinant Streptomyces lividans strain was investigated. The bacterium produced several general peptidases and tripeptidyl peptidases compromising the authenticity of rhIL-3. The level of peptidases depended on growth morphology. Growing S. lividans as compact pellets successfully reduced peptidase activity. Maximum general peptidase activity in pellet culture was delayed after maximum rhIL-3 concentration was achieved. The activity of the tripeptidyl peptidase was product (rhIL-3) associated.
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