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  • Title: Novel cross-linked polyacrylamide matrices: an investigation using gradient gel electrophoresis.
    Author: Caulfield MJ, Purss HK, Solomon DH.
    Journal: Electrophoresis; 2001 Dec; 22(20):4297-302. PubMed ID: 11824593.
    Abstract:
    Gradient gel electrophoresis was used to examine the separation properties of novel cross-linking compounds for polyacrylamide (PAAm). At low %T and at the same %C protein migration difference is accentuated for bismethacrylamide cross-linked networks relative to bisacrylamide cross-linked networks. Similar properties were observed for cyclic monomers at low %T. This trend is maintained throughout the gradient. However, at higher %T migration differential relative to N,N'-methylenebisacrylamide (Bis) was less pronounced. Evidence from gradient gels suggests that reactivity and functionality of vinyl groups impose an overriding control over network formation.
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