These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [A comparison of polymerase chain reaction with other two widely used methods in detection of Porphyromonas gingivalis].
    Author: Jin X, Yang S, Wang S.
    Journal: Zhonghua Kou Qiang Yi Xue Za Zhi; 2000 Jan; 35(1):38-40. PubMed ID: 11831961.
    Abstract:
    OBJECTIVE: To compare the efficiency of polymerase chain reaction (PCR) with cultural method and indirect immunofluorescence (IF) in detection of Porphyromonas gingivalis (P.g) in oral plaque samples. METHODS: 92 subgingival plaque samples were collected from patients of three kinds of periodontal disease. A program using PCR to detect Fimbrilin gene (Fim A) was designed to detect P.g. The efficiency of this PCR method was compared with cultural method and IF. RESULTS: The positive-rate of PCR was 94.6%, while that of cultural method and IF was only 48.9% and 68.5% respectively. The difference was significant in statistical analysis (P < 0.001). CONCLUSION: PCR is more sensitive than culture method and IF in detecting pathogens of periodontal diseases. The study provides a simplified and efficient method for oral clinical use.
    [Abstract] [Full Text] [Related] [New Search]