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  • Title: [An electron microscopic histochemical study on the fibrous formation in pleomorphic adenoma of the salivary gland].
    Author: Wang J, Zhao Y, Tang Q.
    Journal: Zhonghua Kou Qiang Yi Xue Za Zhi; 2000 Jan; 35(1):50-1. PubMed ID: 11831965.
    Abstract:
    OBJECTIVE: To study the kinds of fibers and mechanism of fibrous formation in myxochondroid tissue of pleomorphic adenoma. METHODS: Eight cases of pleomorphic adenomas of salivary gland were studied by histological and electron microscopic histochemical techniques. RESULTS: The histological study showed that collagenous fibers and elastic fibers which constituted the trestle were distributed in myxochondroid areas of pleomorphic adenoma. The electron microscopic histochemical study showed that the myxochondroid tissue contained slender collagen fibrils measuring 50 nm in diameter and heavily stained elastic fibers. In cytoplasm of the myxochondroid cells two types of the Golgi-derived vesicles were seen: one was small round vesicles with a uniform electron dense core of amorphous material and heavily stained; another type was small round vesicles with a non-uniform electron dense core of filamentous material and stained grey. These materials were released into the extracellular space by cytoplasmic exocytosis. CONCLUSIONS: Neoplastic myoepithelial cells have the ability to synthesize and secrete procollagen and precursor of elastin, thus forming collagenous fibers and elastic fibers in myxochondroid areas.
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