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  • Title: Directed mutagenesis and plasmid-based complementation in the methanogenic archaeon Methanosarcina acetivorans C2A demonstrated by genetic analysis of proline biosynthesis.
    Author: Zhang JK, White AK, Kuettner HC, Boccazzi P, Metcalf WW.
    Journal: J Bacteriol; 2002 Mar; 184(5):1449-54. PubMed ID: 11844777.
    Abstract:
    We report here the first use of directed mutagenesis in Methanosarcina acetivorans C2A. The method employs homologous recombination-mediated gene replacement and was used to construct a variety of proline auxotrophs with mutations in the proABC locus. Each mutation was also complemented in trans with autonomously replicating Methanosarcina-Escherichia plasmid shuttle vectors.
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