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  • Title: Cholesterol oxidase. Further studies of substrate specificity in relation to the analytical characterisation of steroids.
    Author: Brooks CJ, Smith AG.
    Journal: J Chromatogr; 1975 Oct 29; 112():499-511. PubMed ID: 1184684.
    Abstract:
    The substrate specificity of cholesterol oxidase has been further examined with respect to the size and shape of the steroid 17beta-side-chain and oxygenation of the nucleus. The relatively unreactive 17-keto-delta5-3beta-hydroxysteroids were converted to satisfactory substrates by formation of the isopentyl- or benzyloximes. The results obtained have been used to illustrate further uses of the enzyme in the gas-phase analytical characterisation and selective oxidation steroids. Oxidations were studied by kinetic experiments, gas-liquid chromatography-mass spectrometry.
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