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  • Title: Application of open-tubular column/gas-liquid chromatography to the analysis of complex mixtures of branched-chain fatty acids.
    Author: Smith A, Lough AK.
    Journal: J Chromatogr Sci; 1975 Oct; 13(10):487-90. PubMed ID: 1184715.
    Abstract:
    Methyl and butyl esters of branched-chain fatty acids of subcutaneous triacylgycerols from barley-fed lambs were subjected to urea-adduction and the resulting fractions were analyzed as methyl esters by gas-liquid chromatography on wall-coated open-tubular columns. The chromatogram of each fraction was less complex than that of the original and there was evidence to suggest that, in the original mixture of branched-chain acids, components resistant to urea-adduction were present, but their occurrence was obscured in chromatograms by the presence of monomethyl-substituted acids.
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