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  • Title: [Characterization of a panel of monoclonal antibodies to hepatitis C NS3 recombinant protein ].
    Author: Abdulmedzhidova AG, Masalova OV, Atanadze SN, Ulanova TI, Burkov AN, Khudiakov IuE, Fields H, Kushch AA.
    Journal: Vopr Virusol; 2002; 47(1):21-5. PubMed ID: 11852778.
    Abstract:
    Recombinant protein rNS3 imitating helicase region (1356-1459 amino acid residues) of hepatitis C virus (HCV) was expressed in E. coli cells and used for BALB/c mice immunization. Seven hybrydoma clones producing monoclonal antibodies (MAbs) to rHS3 were obtained. All MAbs reacted in ELISA with NS3 protein from Murex anti-HCV Version III and in immunoblotting from RIBA 3. These MAbs detect 5 individual epitopes, 4 of which were conformational and 1 discontinuous. All MAbs could compete for rNS3 binding with serum antibodies from patients with chronic hepatitis C, which suggests that these MAbs can recognize the natural HCV NS3 protein.
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